Oxytocin is a naturally occurring hormone common to many mammalian species, including humans. It is a peptide composed of nine amino acids which is synthesized in the hypothalamus and released from the pituitary. One of the main roles of oxytocin is to stimulate contractions during child birth, hence its name, which derives from the Greek phrase “quick birth.”

The oxytocin molecule.

The interesting thing about oxytocin is that it is one of those amazing chemicals which affect mood and social behaviour. It is sometimes referred to as the “cuddle hormone” because elevated levels in the nervous system induce feelings of trust and affection between individuals. Release of oxytocin from the brain appears to be connected with the development of social groups, mother-child bonding and bonding between couples.

20+ students from Kildonan East Collegiate toured the life sciences facilities (Pharma, QA, and CBST) at Red River College.  They then participated in an Eppendorf standardization exercise with CBST faculty and discovered that analytical precision can be a challenge indeed. In addition, they also determined that a number of samples were spiked to ensure analyst integrity.  Prizes were awarded and report having a good time.

The Chemistry/Bioscience students are away this month, working hard at their respective co-op placements. We hope to soon bring you some news about the work our students are doing, but, in the interim and just for fun, here’s some interesting information that many people

Canada’s Black Brant rocket

don’t know about science in Manitoba.

Nestled among the canola fields of the Rockwood municipality a half hour north of Winnipeg is the Bristol Aerospace Propellant Plant. This plant is the only large scale commercial manufacturer of rockets in Canada. Opened in 1962, the plant makes a wide variety of solid propellant rockets, including the CRV7, the CL289 and the Black Brant. The Black Brant (pictured) is a so-called “sounding” rocket, used to explore atmospheric phenomena. It has been in production at the plant for decades and is still used by NASA. When combined with other stages, the Black Brant can reach altitudes of over 1000 km, allowing payloads to experience periods of microgravity or follow chemical reactions in the upper atmosphere.

As with the other Bristol rockets, the Black Brant is powered by the industry standard AP/HTPB propellant based on ammonium perchlorate mixed with polybutadiene. The Brant is particularly popular with researchers due to its very high reliability: over 800 have been launched over the years, with a 98% success rate!

On June 6th, the local chapter of the AOAC (the Association of Official Analytical Communities) will hold its annual “AOAC Day” in Winnipeg, at the Victoria Inn on Wellington. The day will feature technical presentations, free training sessions, equipment displays, a buffet lunch and much more, including a fascinating talk by Dr. Ed Cloutis of the University of Winnipeg, entitled “Exploring Mars with the Curiosity rover: analytical capabilities and latest results.” Registration is only $25 at the door. For more information about the local AOAC and AOAC day, follow this link.

As well, you can find the latest (May 2013) AOAC newsletter here.

Students working on preparing samples for their HPLC analysis

One group of second year students from the Chemical and Biosciences Technology program continued the research started by faculty members Michael Judge and Curtis Aab using Ethyl Lactate (EL) as an HPLC solvent. This work was conducted as part of the new Research Project course recently added to the program. The students explored the use of EL with a new analyte which was methocarbamol , an over the counter (OTC) drug used to treat back pain.

As part of their work the students developed a research proposal, provided ongoing updates of their results, and prepared a final report and presentation. The research was successful as they were able to analyze a finished product, Robaxacet that contained both methocarbamol and acetaminophen. As a starting point the students modified an existing method from the USP (United States Pharmacopeia) for analyzing methocarbamol. They increased the efficiency of this method because the EL mobile phase yielded a shorter run time while still producing well resolved analyte peaks. This project also created the opportunity for future Chemical and Bioscience Technology students to continue this research next year.

The students have accepted the opportunity to present their research at the 2013 Mid-Canada AOAC day on Thursday, June 6^th, 2013.

The Optima 8000

The Chemical/Biosciences instrumental laboratory recently gained a valuable new tool: a Perkin Elmer Optima 8000 ICP-OES spectrometer. ICP is an acronym for “inductively coupled plasma,” while OES stands for “optical emission spectroscopy.” OES is a method of analyzing chemical mixtures or solutions to determine concentrations of trace elements. In this technique, a sample of the material being analyzed is energized to excite its atoms. Since the excited atoms of each element emit light at specific characteristic wavelengths, the presence and the amounts of various elements can be determined by monitoring the emitted light. Some instruments use the heat of a flame to excite the sample, but ICP uses a “plasma” made of ionized argon gas energized by an electromagnetic field.

One of the most important features of an ICP instrument is its very low detection limit. An ICP can typically detect the presence of analytes, such as arsenic or lead in drinking water, at concentrations below one part per billion. When you consider that one part per billion is the equivalent of one second out of a time span of 32 years, the amazing ability of this instrument becomes obvious.

The department is excited about having this new instrument on board and to be able to allow students to gain some valuable hands-on experience in this state-of-the-art analytical technology.

Solvatochromism was used to determine the polarity of ethyl lactate.

Red River College encourages instructional staff to undertake original research and, last year, faculty in the chemical and biosciences program spent some time looking at ways to improve a common analytical method. High performance liquid chromatography, or HPLC, is very widely used in chemical labs and processing facilities to separate and analyze chemical mixtures. However, most HPLC systems employ liquid solvents, such as methanol, which are often toxic.

Curtis Aab and Michael Judge were curious as to whether these toxic solvents could be replaced with a different, more environmentally-friendly liquid. They used ethyl lactate, a solvent which is so safe that it can be applied as an ingredient in cosmetics and foods. Research at the College demonstrated that ethyl lactate could indeed replace methanol when analyzing different pharmaceutical compounds using an officially approved HPLC method. Subsequent work by chemical/biosciences students extended the range of pharmaceuticals which can be analyzed using ethyl lactate. These findings could potentially allow at least some of the thousands of HPLC systems worldwide to transition to a “greener” process.

The research results were accepted for publication in the Canadian Journal of Chemistry and appeared in the May 2013 issue under the title “Ethyl lactate as an environmentally friendly HPLC mobile-phase modifier in the analysis of acetaminophen, caffeine, and ASA.” A link to the journal website is provided below.

http://www.nrcresearchpress.com/journal/cjc

A student discusses dye oxidation results.

This April, the second year students of the Chemical and Biosciences Co-op diploma program presented the results of their independent research projects, representing many hours of hard work over the preceding four months. Each group of students worked independently under the supervision of a faculty member to perform a unique project designed to investigate various aspects of chemical or biological sciences. The projects were extremely varied and represented the wide array of skills taught in the program. The topics investigated were:

• Testing rice for arsenic using inductively coupled plasma – optical emission spectroscopy
• Environmentally-friendly oxidation of dyes in wastewater via tungstate-catalyzed peroxide
• Antimicrobial properties of plant extracts
• Genetically modified foods in common grocery products
• Rates of methicillin-resistant bacteria in the general population
• A “green” solvent for use in liquid chromatography

Students presented a number of surprising and interesting findings, including evidence that at least one brand of “organic” snack foods contained genetically modified corn. Some of the student groups may go on to present their work at the upcoming Association of Analytical Communities (AOAC) event, June 6th at the Victoria Inn, right here in Winnipeg.

Please join us for displays and a pizza lunch in the library hallway May 6th and 7th, as our students and faculty showcase Nursing: A Leading Force for Change.

The College of Registered Nurses of Manitoba (CRNM) will be honouring two of our nursing faculty with awards on Thursday May 9. Winn Briscoe and Leslie Walsh will be presented with the Award of Excellence in Nursing Education.  The award presentation and dinner is at Canad Inns Polo Park from 5:00-9:00. Tickets are available from pyeates@crnm.mb.ca or (204) 789-0662. No reserved seating. The order deadline is May 2nd.

Congratulations Winn and Leslie!